Analysis Note

ControlPositive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.

Application

Research Sub CategoryEpitope Tags

Research CategoryEpitope Tags & General Use

Anti-Myc Tag Antibody, clone 4A6, HRP conjugate detects level of Myc Tag & has been published & validated for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Myc-Tag is a short synthetic peptide sequence derived from C-terminal region of human c-myc that is used as an epitope tag. The Myc tag can be cloned either upstream or downstream of the target gene, resulting in a fusion protein that allows efficient purification, detection, and localization of target proteins. The relatively small size of this tag vs. other commonly used tags such as GST (30 kDa) makes it less likely to interfere with target protein structure or function. The myc epitope tag is widely used to detect expression of recombinant proteins in bacterial, yeast, insect, and mammalian systems.

Immunogen: peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc

Specificity: Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.

Species Cross-reactivity: Human, others not tested

Ig Type: IgG

Host: Mouse

Molecular Weight: Varies depending upon the protein which is tagged

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc. Clone 4A6.

Epitope: C-terminal

Legal Information

Kathon is a registered trademark of Rohm and Haas Co.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal IgG1 conjugated to horseradish peroxidase (HRP) in buffer containing: 50% storage buffer (PBS, 0.05% Kathon^®, 0.2 mg/mL BSA) and 50% glycerol.

Quality

Evaluated by Western Blot on Myc-PP2A A subunit transfected NIH/3T3 lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected myc-PP2A on 10 µg of Myc-PP2A A subunit transfected NIH/3T3 lysates.

Specificity

Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.

Other species cross-reactivity not tested.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Target description

Varies depending upon the protein which is tagged.